Conformational study reveals amino acid residues essential for hemagglutinating and anti-proliferative activities of Clematis montana lectin

Conformational study reveals amino acid residues essential for hemagglutinating and anti-proliferative activities of Clematis montana lectin.

Clematis montana lectin (CML), a novel mannose-binding lectin purified from C. montana Buch.-Ham stem (Ranunculaceae), has been proved to have hemagglutinating activity in rabbit erythrocytes and apoptosis-inducing activity in tumor cells. However, the biochemical properties of CML have not revealed and its structural information still needs to be elucidated. In this study, it was found that CM...

Evaluation of linoleic acid, eladic acid and palmitic acid for cytotoxicity and anti-inflammatory activities

Background and objectives: Nigella sativa seeds have shown to possess anti-inflammatory, anti-oxidant, trenquillizer, chemopreventive and anti-neoplastic effects. Besides, they areused as nutritional flavoring agents. We have investigated the protective effects of N.  sativa seeds hexane extract and active principles against methyl phenyl pyridine (MP...

Amino acid residues modulating the activities of staphylococcal glutamyl endopeptidases.

The glutamyl endopeptidase family of enzymes from staphylococci has been shown to be important virulence determinants of pathogenic family members, such as Staphylococcus aureus. Previous studies have identified the N-terminus and residues from positions 185-195 as potentially important regions that determine the activity of three members of the family. Cloning and sequencing of the new family ...

Effects of denaturation and amino acid modification on fluorescence spectrum and hemagglutinating activity of Hericium erinaceum Lectin.

A sialic acid-binding lectin (Hericium erinaceum lectin, HEL), isolated from fresh fruiting bodies of Hericium erinaceum, was treated with various temperature and pH to investigate its fluorescence spectra and hemagglutinating activity. It was found that the hemagglutinating activity of HEL was relatively steady below 60 degrees and at pH from 6 to 11, and the change of hemagglutinating activit...

anti-proliferative and apoptotic activities of chrysin in neuroblastoma cells